Botox Injections: Botox is a protein produced by the bacterium Clostridium botulinum. It binds to nerve endings preventing muscles from contracting. Since wrinkles result from muscular contraction, BOTOX injections virtually eliminate wrinkles for the life of the BOTOX (usually 3-5 months). BOTOX is most effective for forehead wrinkles, crow's feet around the eyes, and frown lines.